Fig. 1. miR-675-3' is the functional arm in adult human articular chondrocytes. A-D, RT-qPCR for miR-675-5' and 3' (A), miR-140-5' and 3' (C) and their respective host gene H19 (B) and WWP2 (D) in input fractions and after immunoprecipitation with AGO2 antibody. miR levels were determined by TaqMan technology and genes were analysed by SYBR Green. Statistical analysis was carried out with a paired, two-tailed t-test. In, input; AGO2, IP with AGO2; Ig, IP with IgG; IP, immunoprecipitation.